Growth and enterotoxin B synthesis by Staphylococcus aureus S6 in associative growth with Pseudomonas aeruginosa

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Abstract
The interaction of Pseudomonas aeruginosa and Staphylococcus aureus S6 was studied in two systems. In the first system, the two organisms were grown together in a single flask. Growth of P. aeruginosa was unaffected, but growth of S. aureus was modified. After 24 h, 99.9% of the staphylococci population lost their salt tolerance when plated on media containing 7.5% sodium chloride, and enterotoxin B synthesis by S. aureus was diminished. In the second growth system, pure cultures of P. aeruginosa and S. aureus were grown in membrane-type spinner flasks. The growth and salt tolerance of S. aureus was again affected, but to a lesser degree. Cultures of S. aureus from these experiments recovered their salt tolerance in 6 h when transferred to fresh medium.Nutrient deficiency, lack of oxygen, or pigment production by the pseudomonads did not contribute significantly to loss of salt tolerance or inhibition of enterotoxin B synthesis, but a staphylolytic enzyme(s) isolated from P. aeruginosa was shown to be responsible for the loss of these properties.

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