Melittin Stimulates Incorporation and Degradation of Sphingomyelin in Synaptosomal Plasma Membranes
- 1 May 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 38 (5) , 1230-1235
- https://doi.org/10.1111/j.1471-4159.1982.tb07895.x
Abstract
Melittin enhanced sphingomyelin (SPM) degradation by the neutral membrane‐bound sphingomyelinase from calf brain synaptosomal plasma membranes (SYM) up to 20‐fold. Melittin in concentrations as high as 100 μM did not significantly alter membrane fluidity of SYM as measured by fluorescence depolarization and electron spin resonance (ESR) using diphenylhexatriene and a doxy1 derivative of SPM, respectively. In the concentration range 100‐1000 μM. melittin was observed to rigidify SYM. The incorporation of SPM.erivatives into the lipid bilayer of SYM.as demonstrated by ESR measurements. Melittin enhanced the uptake of SPM‐derivatives into SYM.Keywords
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