UPTAKE OF 5‐HYDROXYTRYPTAMINE BY RAT BLOOD PLATELETS AND ITS INHIBITION BY ADENOSINE 5‘‐DIPHOSPHATE
Open Access
- 1 April 1976
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 56 (4) , 417-421
- https://doi.org/10.1111/j.1476-5381.1976.tb07452.x
Abstract
1 The uptake of 5-hydroxytryptamine (5-HT) by rat blood platelets in citrated plasma was linear for at most 10 s and was substantially complete within 3 minutes. 2 Adenosine 5′-diphosphate (ADP) was a potent inhibitor of 5-HT uptake (Ki = 0.38 μm) and kinetic analysis revealed that the inhibition was not competitive. 3 Inhibition of 5-HT uptake by ADP was abolished in the presence of prostaglandin E1 and 2-n-amylthio-AMP, which also inhibit the stimulant actions of ADP on blood platelets. 4 It is concluded that ADP could inhibit 5-HT uptake by changing the Na+/K+ distribution across the cell membrane, and the biological significance of this is discussed.Keywords
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