A monoclonal antibody toBabesia divergenswhich inhibits merozoite invasion
- 1 February 1987
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 94 (1) , 17-27
- https://doi.org/10.1017/s0031182000053415
Abstract
SUMMARY: A monoclonal antibody-producing hybridoma cell line has been raised against merozoites ofBabesia divergens. This antibody is strongly reactive against merozoites in an enzyme-linked immunosorbent assay (ELISA) and is also positive in an indirect fluorescent antibody test (IFAT) on preparations of live merozoites. The antibody recognizes an antigen, of molecular weight (Mr) 50–60 K by Western blot analysis on SDS–polyacrylamide gel electrophoresis (SDS–PAGE) gels. Merozoite neutralization assays show that the antibody significantly inhibits merozoite invasion of bovine erythrocytes.Keywords
This publication has 22 references indexed in Scilit:
- Vaccination with purified microgamete antigens prevents transmission of rodent malariaNature, 1985
- Immune responses of cattle following vaccination with living and non-living Babesia bovis antigensVeterinary Parasitology, 1984
- Research on babesiosis vaccinesPublished by Springer Nature ,1984
- Continuous in Vitro Cultivation of Babesia DivergensActa Veterinaria Scandinavica, 1982
- Bovine Babesiosis: Protection of Cattle with Culture-Derived Soluble Babesia bovis AntigenScience, 1981
- Babesia bovis : Continuous Cultivation in a Microaerophilous Stationary Phase CultureScience, 1980
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- Babesia argentina: Immunogenicity of plasma from infected animalsExperimental Parasitology, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Bovine babesiosis: The immunization of cattle with killed Babesia argentinaExperimental Parasitology, 1967