Reduction of Voltage-Dependent Mg 2+ Blockade of NMDA Current in Mechanically Injured Neurons
- 13 December 1996
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 274 (5294) , 1921-1923
- https://doi.org/10.1126/science.274.5294.1921
Abstract
Activation of the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors is implicated in the pathophysiology of traumatic brain injury. Here, the effects of mechanical injury on the voltage-dependent magnesium (Mg2+) block of NMDA currents in cultured rat cortical neurons were examined. Stretch-induced injury was found to reduce the Mg2+ blockade, resulting in significantly larger ionic currents and increases in intracellular free calcium (Ca2+) concentration after NMDA stimulation of injured neurons. The Mg2+ blockade was partially restored by increased extracellular Mg2+ concentration or by pretreatment with the protein kinase C inhibitor calphostin C. These findings could account for the secondary pathological changes associated with traumatic brain injury.Keywords
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