Elektrophoretische Untersuchungen an Mutanten des Tabakmosaikvirus

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Abstract
Electrophoretic pH-mobility diagrams were constructed for two groups of TMV strains. In each group the strains followed each other by single or by few mutation steps. In accordance with biological symptoms and serological results, the division into two groups could be seen also in electrophoretic behaviour (differences in number of electrophoretically active basic components). There is no parallelism however between alterations of biological symptoms and electrophoretic mobility. To explain the differences in electrophoretic behaviour of our TMV strains it is suggested that only the outermost part of the protein coat should be considered as electrophoretically active. Thus differences in mobility could be caused not only by alterations in amino acid content but also by changes in the distribution of the charge-defining groups at the virus surface — e. g. the sequence of amino acids in the peptid chain.

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