Studies on Phenolic Steroids in Human Subjects. XVI. Role of the Kidney in the Disposition of Estriol*

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Abstract
Results obtained in human subjects following the administration of labeled estriol by single injection or continuous infusion into a renal artery and a peripheral vein indicated strongly that the human kidney possesses the capability to 16-glucosiduronidate this steroid Hence, homogenates of human kidney were incubated with estriol and UDPGA and facile glucosiduronidation of the steroid was demonstrated. The conjugate was shown to be the 16α-glucosiduronate by its identity with standard compoundin its behavior in countercurrent distribution (CCD), chromatography, and following various hydrolytic procedures. To extend the human studies, baboon kidney was also shown to be capable of glucosiduronation of estriol, on the basis of estriol-16α-glucosiduronate obtained following the incubation of baboon kidney homogenates and perfusion of the kidney in vitro with estriol. The importance of the 16-glucuronidation of estriol is pointed to by the inability of the human kidney to significantly affect the disposition of estrone or to conjugate it in vitro and of the baboon kidney to significantly glucosiduronate aldosterone. An up-to-date concept of estriol metabolism is presented, taking into consideration the possible important role of the kidney in the disposition of estriol.

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