Embryogenesis and formation of tetraploid and hexaploid plants from carrot protoplasts

Abstract
Carrot cells cultured in vitro as well as protoplasts isolated from these cells formed embryos and grew into mature plants. Treatment of the protoplasts with polyethylene glycol (PEG) did not interfere with the capacity of this material to multiply and differentiate. Plants grown from PEG-treated protoplasts exhibited a higher frequency of tetraploids than those grown from untreated protoplasts and from the original cells.

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