Conformationsl analysis of the trinucleoside diphosphate 3′d(A2′-5′A2′-5′A). An NMR and CD study
Open Access
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (13) , 4569-4582
- https://doi.org/10.1093/nar/11.13.4569
Abstract
A 500 MHz and 300 MHz NMR study of the trinucleoside diphosphate 3′d(A2′-5′A2′-5′A) is presented. In addition, circular dichroism is used to study base stacking in the title compound. The complete 1H-NMR spectral assignment of the sugar ring proton signals is given. Information about the sugar ring (N- or S-type conformation) and about the backbone geometry along C4′-C5′ and C5′-O5′ bonds is obtained from the NMR coupling constants. It is shown that the trimer mainly occurs in the N-N-N stacked state at low temperatures; the presence of a minor amount of N-N-S conformational sequence is indicated.Keywords
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