An Improved Copper Reagent for Quantitative Densitometric Thin-Layer Chromatography of Lipids
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 5 (6) , 1155-1162
- https://doi.org/10.1080/01483918208067575
Abstract
Eleven cupric compounds were screened to determine whether a different cupric anion might give greater and more uniform charring responses with lipid classes on TLC plates than cupric acetate. A procedure was developed which used 10% CuSO4 in 8% H3PO4 as a charring reagent. Improved reproducibility of charring was obtained by temperature-programmed heating of the thin layer plates from 30 to 180°C at 10 degrees per minute in the oven of a commercial gas-liquid chromatograph.This publication has 5 references indexed in Scilit:
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