Evidence that chick tendon procollagen must be denatured to serve as substrate for proline hydroxylase (Short Communication)
- 1 September 1973
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 135 (1) , 249-251
- https://doi.org/10.1042/bj1350249
Abstract
The ability of chick-embryo proline hydroxylase to hydroxylate [14C]proline-labelled procollagen was investigated between 23° and 37°C. The amount of hydroxy[14C]proline that could be formed increased sharply between 26° and 30°C. This corresponded to the temperature interval in which the [14C]procollagen substrate was thermally denatured, and the results therefore indicate that only denatured molecules can be hydroxylated.Keywords
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