Insulin Content of Pancreas after Sodium Fluoroacetate-Induced Hyperglycemia.

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Abstract
A specific immunochemical assay was used to measure extractable insulin from the pancreas of normal rats, alloxan-diabetic rats, and rats made diabetic with sodium fluoroacetate (SFA). Rat insulin proved immuno-logically different from crystalline beef insulin, suggesting a significant chemical difference between these insulins. Pancreatic insulin content was decreased or absent in alloxan-treated diabetic rats, but appeared to be increased in rats with SFA diabetes, compared with normal controls. These findings demonstrate that the diabetes produced by SFA is not due to an alloxan-like action on beta cells resulting in insulin deficiency, but is secondary to a peripheral block in glucose utilization.