A LIGHT MICROSCOPIC METHOD FOR THE DIFFERENTIATION OF NORADRENALINE- AND ADRENALINE-PRODUCING CELLS OF THE RAT ADRENAL MEDULLA

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Abstract
A glutaraldehyde-toluidine blue method is described that allows a differentiation of noradrenaline- from adrenaline-producing cells in paraffin-embedded sections of the rat adrenal medulla. The noradrenaline-containing cells stain green, the adrenaline-containing cells purple (β-metachromatic) and the cortical cells blue (orthochromatic). The green staining of the noradrenaline-containing cells can be intensified by chromation of the sections prior to the staining. The green cells have been identified as noradrenaline-producing cells by collateral experiments. The method is simple and reliable and should prove generally useful.

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