Chloride Transport across Isolated Frog Skin

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Abstract
Kristensen, P. Chloride transport across isolated frog skin. Acta physiol. scand. 1972. 84. 338–346.A net inward transport of chloride was found to occur in isolated skins of Rana temporia. This transport was not dependent on sodium transport and was inhibited by diamox, thio‐cyanate and bromide. The active chloride transport was not affected by changes in the transepithelial potential difference. On the other hand, the curve describing efflux as a function of potential difference was found to have a shape resembling the shape of the Goldman equation.Graphical determination of apparent pools showed the influx pool to be about six times greater than the efflux pool. Diamox had no effect on efflux pool size but reduces the apparent influx pool so that it becomes about equal to the efflux pool. This means that at least part of the paths for active and passive chloride movements are different.A working hypothesis is put forward, according to which chloride is actively transported across the outward facing membrane of the outermost living cell layer of the frog skin epithelium, and the main part of the passive flux occurs across the tight seals and via the interspace system.