Preservation of Human Tracheal Organ Cultures at - 196 C
- 1 February 1973
- journal article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 6 (1) , 123-126
- https://doi.org/10.1099/00222615-6-1-123
Abstract
Human embryonic tracheal organ cultures have been frozen to — 196°C and thawed without any apparent loss of ciliary activity or susceptibility to the growth of human influenza virus. Optimal preservation was obtained by freezing in the presence of 25 per cent. dimethylsulphoxide at a cooling rate of 0·3°C per min. This extremely simple technique facilitates the storage of tracheal organ cultures for experimental use.Keywords
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