Effect of the Removal of Outer Cell Wall Layers on the Actinomycin Susceptibility of a Gram-Negative Bacterium
Open Access
- 1 May 1972
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 1 (5) , 447-449
- https://doi.org/10.1128/aac.1.5.447
Abstract
Removal of the outer cell wall layers of a gram-negative marine pseudomonad (B-16) showed that these cells are penetrable by actinomycin D and that, therefore, neither the cytoplasmic membrane nor the peptidoglycan layer constitutes the barrier which excludes this antibiotic from intact cells, but that this barrier is formed by the outer layers of the cell wall which include the lipopolysaccharide component and the double-track layer.Keywords
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