NADH oxidation in submitochondrial particles protects respiratory chain activity against damage by adriamycin‐Fe3+
- 1 December 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 137 (1-2) , 113-118
- https://doi.org/10.1111/j.1432-1033.1983.tb07803.x
Abstract
Oxidative decomposition of polyunsaturated fatty acid moieties of membrane lipid in pig heart submitochondrial particles, as initiated by ferric ion complexes of the antineoplastic drug adriamycin and concomitant inactivation of oxidase activities, is counteracted by EDTA, low oxygen pressure, a phenolic antioxidant and NADH oxidation through the respiratory chain but not by scavengers of reactive oxygen species. Protection by NADH is strengthened by removal of cytochrome c from the submitochondrial particles and by antimycin A but abolished by rotenone.Inhibition of cytochrome c oxidase activity by the adriamycin‐Fe3+ complex is reversible and activity is recovered upon cholate solubilization of the particles. ADP inhibits binding of the complex to the submitochondrial particles and protects both cytochrome c oxidase activity and membrane lipid.The results are discussed in relation to the possible role of mitochondrial function in protection against free‐radical‐mediated effects of adriamycin.This publication has 47 references indexed in Scilit:
- A ‘hexokinase effect’ in the inhibition of oxidative phosphorylation in heart muscle mitochondria by AdriamycinBiochemical and Biophysical Research Communications, 1982
- Adriamycin inactivates cytochrome c oxidase by exclusion of the enzyme from its cardiolipin essential environmentBiochemical and Biophysical Research Communications, 1982
- Decompostion of unsaturated phospholipid by iron-ADP-adriamycin co-ordination complexBiochemical and Biophysical Research Communications, 1981
- Solution chemistry studies of adriamycin-iron complexes present in vivoPublished by Elsevier ,1980
- Adriamycin stimulated superoxide formation in submitochondrial particlesChemico-Biological Interactions, 1977
- Adriamycin: The Role of Lipid Peroxidation in Cardiac Toxicity and Tumor ResponseScience, 1977
- Generation of free radicals and lipid peroxidation by redox cycling of adriamycin and daunomycinBiochemical and Biophysical Research Communications, 1977
- Strand scission of DNA by bound adriamycin and daunorubicin in the presence of reducing agentsBiochemical and Biophysical Research Communications, 1977
- Inhibition of coenzyme Q10-enzymes, succinoxidase and NADH-oxidase, by adriamycin and other quinones having antitumor activityBiochemical and Biophysical Research Communications, 1974
- The Kinetics of the Redox Reactions of Ubiquinone Related to the Electron‐Transport Activity in the Respiratory ChainEuropean Journal of Biochemistry, 1973