Rapid proteolysis of brain MAP-1 related cytoskeleton-associated 350kd protein by purified calpain.
Open Access
- 1 January 1986
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 11 (3) , 253-257
- https://doi.org/10.1247/csf.11.253
Abstract
Microtubule associated protein-1 of brain and its intracellular 350kd analogues were highly sensitive to purified Ca2+-dependent cysteine proteinase (calpain). After 15 second digestion, we detected intermediate degradation products of MAP-1 by immunoblotting using anti-MAP-1 antibody as 290, 260, 220, 170, 140, 112, 80, 68, and 32kd polypeptides. These values corresponded to the molecular weights of the immunoreactive polypeptides of microtubule-enriched cytoskeletons isolated from HeLa and SV-3Y1 cells, suggesting the action of endogenous calpain on intracellular MAP-1 analogues in vivo or during the course of preparation.This publication has 14 references indexed in Scilit:
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