T-LYMPHOCYTES AND B-LYMPHOCYTES AND REED-STERNBERG CELLS IN HODGKINS-DISEASE LYMPH-NODES AND SPLEENS

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 24  (2) , 280-286
Abstract
Lymphoid cells from 24 untreated Hodgkin''s disease biopsies were examined for spontaneous sheep erythrocyte and sensitized ox erythrocyte rosette formation for the identification of T [thymus-derived] cell and cells with Fc and C3 [3rd component of complement] receptors and surface immunoglobulin. Compared with normal tissues mean T-lymphocyte values were elevated in involved lymph nodes and uninvolved spleens from Hodgkin''s patients. Lymphocytes bearing C3 receptors were correspondingly reduced in these tissues. Involved spleen T-cell values fell within the normal range. In normal tissues the sum of lymphocytes with surface immunoglobulin and sheep erythrocyte receptors fell in the range 89-108%. In 6 biopsies of Hodgkin''s tissue the sum was outside the normal range (121-142%). This observation is compatible with surface immunoglobulin-coated T cells. Surface marker characteristics and intracellular immunoglobulin studies of small lymphocytes, lymphoblasts and Hodgkin''s cells suggested that the neoplastic cells were of B [bone marrow-derived] lymphocyte origin.