A MONOCLONAL-ANTIBODY WITH SPECIFICITY FOR TRYPANOSOMA-CRUZI, CENTRAL AND PERIPHERAL NEURONS AND GLIA

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 54  (3) , 617-624
Abstract
Of 26 hybridomas secreting anti-T. cruzi antibodies, 2 reacted with murine brain and spinal cord extracts but not other tissues. Both antibodies (5H7 and 3H3) had identical isotypes (IgM) and specificities as judged by western blotting. Antigens of MW 58,000 and 37,000 in mouse brain and spinal cord, and 58,000 and 35,000 in T. cruzi were detected. Different tissue antigens were recognized by a previously reported T. cruzi mammalian neural tissue cross-reacting monoclonal antibody CE5 on immunofluorescence 5H7 stained some glia, and some central and peripheral neurons in rat tissue sections. Pooled sera from mice chronically infected with T. cruzi competed with binding of 5H7 to T. cruzi antigens.