Effect of source of calcium and phosphate, autoclaving, vitamin D3level and strain of turkey poults on the rachitogenic activity of isolated soybean protein1

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Abstract
This study was conducted to ascertain the effect of autoclaving, source of calcium and phosphate, vitamin D3 level and strain of turkey poults on the rachitogenic activity of isolated soybean protein (C‐1 protein‐Skidmore). One‐day‐old poults were fed purified diets of the glucose—C‐1 protein type. The diets contained calcium, phosphorus and vitamin D3 at levels equal to or greater than the National Research Council's (1960) recommendations. The findings demonstrate that USP hydrated dicalcium phosphate was more effective than food‐grade dicalcium phosphate in overcoming the rachitogenic activity of the C‐1 protein. Autoclaving the C‐1 protein for 80 min destroyed its rachitogenic activity: Vitamin D3 was also effective in reducing the rachitogenic effect of C‐1 protein. The Broad Breasted White strain proved to be more susceptible to the rachitogenic property of C‐1 protein than the Wrolstad Small White strain. Substituting USP hydrated dicalcium phosphate for food‐grade dicalcium phosphate, autoclaving the C‐1 protein or increasing the vitamin D3 level increased body weight gains and tibia ash values. The interactions which were shown to exist between source of calcium and phosphate × autoclaving, strain × autoclaving, and vitamin D3 level × strain demonstrate an interrelationship between these factors.