Ti plasmid and chromosomal ornithine catabolism genes of Agrobacterium tumefaciens C58
- 1 July 1983
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 155 (1) , 196-202
- https://doi.org/10.1128/jb.155.1.196-202.1983
Abstract
The pTiC58 plasmid noc genes of A. tumefaciens C58 code for nopaline oxidase (nocC), the inducible periplasmic protein n1 (nocB) and a function(s) required for ornithine catabolism (nocA). Strains C58 and Ach-5 of A. tumefaciens have chromosomal ornithine catabolism genes. The chromosomal orc gene codes for ornithine dehydrogenase. Strain C58 is normally orc, but orc+ mutants can be selected. Chromosomal orc and pTiC58 nocA plasmid genes were characterized. Complementation of most chromosomal orc mutants by pTiC58 restored growth on nopaline and L-ornithine but did not restore ornithine dehydrogenase activity. Ornithine is an intermediate of nopaline degradation and the Ti plasmid and chromosomes code for ornithine-degradative enzymes. A model for nopaline catabolism is presented.This publication has 32 references indexed in Scilit:
- Internal organization, boundaries and integration of Ti-plasmid DNA in nopaline crown gall tumoursJournal of Molecular Biology, 1980
- Studies on Agrobacterium tumefaciens. VII. Avirulence induced by temperature and ethidium bromideCanadian Journal of Microbiology, 1977
- Inducible Permease Involved in the Uptake of Octopine, Lysopine and Octopinic Acid by Agrobacterium tumefaciens Strains Carrying Virulence-associated PlasmidsJournal of General Microbiology, 1977
- A useful synthesis of nopaline, a crown gall tumor metaboliteBiochemical and Biophysical Research Communications, 1977
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Biodegradative ornithine decarboxylase of Escherichia coli. Purification, properties, and pyridoxal 5'-phosphate binding siteBiochemistry, 1975
- Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing abilityNature, 1974
- Supercoiled circular DNA in crown-gall inducing Agrobacterium strainsJournal of Molecular Biology, 1974
- Degradation of lysopine by an inducible membrane‐bound oxidase in Agrobacterium tumefaciensFEBS Letters, 1972
- Studies on Agrobacterium tumefaciens: characterization of strains 1D135 and B6, and analysis of the bacterial chromosome, transfer RNA and ribosomes for tumor-inducing abilityPhysiological Plant Pathology, 1972