Synthesis of Ribosomal and Transfer Ribonucleic Acids in Yeast During a Nutritional Shift-up

Publisher Website
Google Scholar
Abstract
The growth rate of Saccharomyces cerevisiae was increased by adding a mixture of amino acids to cultures containing proline as the sole N source. The transition from balanced growth in the basal medium (doubling time 4 h) to balanced growth in the enriched medium (doubling time 2 h) took about 2.5 h. The rate of RNA accumulation increased soon after the enrichment to almost its final value. This increase began after a short lag of 10-15 min, therefore synthesis of new RNA polymerase molecules may be required before stable RNA production can increase. The different stable RNA species were not stimulated at different times after the enrichment, but all increased continuously throughout the transition. The rRNA species accumulated in a co-ordinate fashion at a rate faster than the rate of tRNA accumulation.

This publication has 1 reference indexed in Scilit: