THE MEMBRANE FILTER IN MARINE MICROBIOLOGY
- 1 December 1952
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 64 (6) , 783-786
- https://doi.org/10.1128/jb.64.6.783-786.1952
Abstract
The membrane filter, employing a filter membrane of cellulose esters and an effective pore size of 0.4 m[mu], effectively retains marine bacteria and has a filtering rate of one liter of water in approx. 5 min. The apparatus is an excellent tool for sampling psychrophylic and other bacteria from sea water. Bacteria retained on the surface of the filter membrane grew into colonies, after a short incubation time, when the opposite side of the filter was placed on an absorbent pad containing nutrient medium. Various nutrient media in coned, form (2-5X) may be used depending on the organisms to be cultivated. The medium diffuses through the filter pores to the bacteria. In an anaerobic environment, it was possible to grow single colonies of sulfate-reducing and anaerobic bacteria on the filter membranes.Keywords
This publication has 6 references indexed in Scilit:
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- HYDROGEN-UTILIZING, SULFATE-REDUCING BACTERIA IN MARINE SEDIMENTSJournal of Bacteriology, 1950
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