Non-Immunogenicity of Purified Hyaluronic Acid Preparations Tested by Passive Cutaneous Anaphylaxis

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Abstract
The immunogenicity of purified hyaluronic acid (HA) from human umbilical cord and rooster comb, and of some modified HA preparations, was studied in rabbits. HA preparations contained 0.28–0.57% of protein or peptides (Folin’s method). Rabbits received four intramuscular injections at 4-weekly intervals of the following potential immunogens, emulsified in complete Freund’s adjuvant: (1) human umbilical cord HA (HUC-HA), 500 μ g/dose; (2) HUC-HA, precipitated with cetylpyridinium chloride (CPC), 500 μ g/dose; (3) HUC-HA-ovalbumin conjugate, precipitated with CPC, 500 μ g/dose, and (4) rooster comb HA at dose levels of 5, 50 and 500 μ g. Rabbit sera were tested before, during, and after immunization for PCA reactive antibodies against HA in guinea pigs. 11 of 25 pre-immunization sera showed low titres of HA-cross-reactive antibodies. No formation of PCA reactive antibodies against any of the four HA preparations was observed during or after immunization.

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