Integration of PCR Fragments at Any Specific Site within Cloning Vectors without the Use of Restriction Enzymes and DNA Ligase
Open Access
- 1 July 2001
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 31 (1) , 88-92
- https://doi.org/10.2144/01311st05
Abstract
Here, we describe a method that offers a unique way to engineer plasmids with precision but without digestion using restriction enzymes for the insertion of DNA. The method allows the insertion of ...Keywords
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