Reaction center and light-harvesting I genes from Rhodopseudomonas capsulata

Publisher Website
Google Scholar
Abstract
Five structural genes coding for the reaction center (RC), L, M, and H subunits and the 2 light-harvesting (LH) I polypeptides, B870.alpha. and B870.beta., were mapped on 2 restriction fragments from the R-prime plasmid pRPS404. It was recently shown that enhanced near-IR fluorescence mutants of R. capsulata typically lack RC or LH I polypeptides and that these lesions are marker-rescued by 2 restriction fragments from the R-prime plasmid: the 7.5-kilobase-pair EcoRI F fragment and the 4.75-kilobase-pair BamHI C-EcoRI fragment. One now determined the nucleotide sequence of 2 restriction fragments and it was found that the BamHI C-EcoRI B fragment carries the structural genes for the RC L and M subunits and both LH I polypeptides. Forty kilobase pairs away from this locus, the BamHI F fragment (within the EcoRI F fragment) carries the RC H subunit. The structural genes on the BamHI C-EcoRI B fragment are probably transcribed as part of a polycistronic mRNA. All of the structural genes begin with a consensus Shine-Dalgarno sequence and separate AUG start codons, indicating that the structural polypeptides are not cleaved from larger precursor polypeptides.

This publication has 12 references indexed in Scilit: