Vibrio cholerae bacteriophage CP-T1: characterization of bacteriophage DNA and restriction analysis

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Abstract
Temperate bacteriophage CP-T1 of V. cholerae has a capsid that is 45 nm in diameter, a contractile tail 65 nm long and 9.5 nm wide and a baseplate with several spikes or short tail fibers. The linear double-stranded DNA is 43.5 .+-. 1.4 kilobases long, and the phage genome is both terminally redundant and partially circularly permuted. The extent of terminal redundancy maps were constructed for the enzymes HindIII, EcoRI, BamHI and PstI. Restriction endonuclease and heteroduplex analyses of phage DNA established the presence and location of a site (pac) at which packaging of phage DNA is initiated.