Elastase in the Exocrine Pancreas: Localization with Fluorescent Antibody
Open Access
- 1 July 1960
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 85 (1) , 78-80
- https://doi.org/10.4049/jimmunol.85.1.78
Abstract
Summary: The enzyme, elastase, was demonstrated in the acinar tissue of the porcine pancreas using antielastase rabbit serum and fluorescent sheep antirabbit globulin as a means of identifying and localizing the enzyme. No elastase was present in the islets of Langerhans. The specificity of the antibody to porcine elastase was demonstrated by its strong reaction with porcine pancreas and its failure to react with human or guinea pig pancreas. A positive reaction occurred between guinea pig anti-insulin serum and the islets of Langerhans of porcine pancreas whereas there was no reaction between this serum and the acinar tissue and ductal epithelium of porcine pancreas.Keywords
This publication has 6 references indexed in Scilit:
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