INVENTORY AND BIOLOGICAL IMPACT OF POLYCYCLIC CARCINOGENS IN THE ENVIRONMENT .18. SCANNING ELECTRON MICROSCOPICAL INVESTIGATIONS ON THE RESPIRATORY EPITHELIUM OF THE SYRIAN GOLDEN-HAMSTER .4. INVITRO EFFECTS OF DIMETHYLSULFOXIDE AND BENZO(A)PYRENE
- 1 January 1982
- journal article
- research article
- Vol. 176 (2-3) , 269-276
Abstract
The biological effects of in vitro exposure of cultured fetal hamster tracheal epithelium to benzo[a]pyrene [BaP] in 0.5% dimethylsulfoxide [DMSO] (BaP: 5 and 10 .mu.g/ml medium) were studied by light microscopy and scanning electron microscopy. The solvent DMSO alone induced a slight, temporary retardation in development and differentiation of cilia. At day 28 of culture, differences in growth and maturity disappeared between DMSO-exposed and untreated explants. No dose-related differences found in the surface morphology of alterations were seen in BaP-treated tracheae; the explants revealed metaplastic foci and reduced, incomplete ciliogenesis compared to DMSO- and nontreated controls. Towards the end of the culture period, ciliogenesis tended slowly to replace epithelial alterations.This publication has 5 references indexed in Scilit:
- Inhalation Studies With Benzo[a]pyrene in Syrian Golden Hamsters23JNCI Journal of the National Cancer Institute, 1981
- Induction of Atypical Squamous Metaplasia with Benzo[a]Pyrene in Cultured Hamster TracheasPathology - Research and Practice, 1980
- Investigations on the carcinogenic burden by air pollution in man. Intratracheal instillation studies with benzo[a]pyrene in a mixture of Tris buffer and saline in Syrian golden hamstersCancer Letters, 1979
- Investigations on the carcinogenic burden by air pollution in man. Intratracheal instillation studies with benzo(a)pyrene in bovine serum albumin in syrian hamstersCancer Letters, 1978
- SCANNING ELECTRON-MICROSCOPY OF MYCOPLASMA-INFECTED TRACHEAL RINGS1976