Improved rapid autoradiography with elimination of background
- 1 April 1985
- journal article
- research article
- Published by Wiley in Cell Biochemistry and Function
- Vol. 3 (2) , 147-148
- https://doi.org/10.1002/cbf.290030211
Abstract
A method has been developed to improve the quality of autoradiography. In addition to using high specific activity thymidine (3HTdr), low temperature exposure and scintillation fluid. FdUR was added to the cell suspension during incubation. It led to an increased amount of 3HTdr incorporation by the cells. By this method, the grain density over positive cells was increased and the background was minimal.Keywords
This publication has 3 references indexed in Scilit:
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- 5-Fluoro-2′-Deoxyuridylate: Covalent Complex with Thymidylate SynthetaseProceedings of the National Academy of Sciences, 1972