Testing the viability of fungal sclerotia

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Abstract
SUMMARY: Simple methods for producing, handling and testing the viability of sclerotia were used on five fungi, three of which produced sclerotia freely in sand‐maizemeal cultures; the other two did not, and were grown on agar. After air drying, which did not affect viability, sclerotia were buried in the field in bags of nylon fabric. After recovery from soil they were surface‐sterilized with calcium hypochlorite and plated on a medium containing chloramphenicol and streptomycin sulphate. Some fungi, particularly those having basidiomycete affinities, are sensitive to high concentrations of chloramphenicol.

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