The use of polystyrene microcarriers to prepare cell monolayers for transmission electron microscopy

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Abstract
A simple method is described, using polystyrene microcarrier beads, that allows cell monolayers to be prepared without distortion for transmission electron microscopy. Cells [human larynx carcinoma HEp2 cell] cultured on polystyrene beads are held in an agar matrix. Before embedding, the beads are dissolved in 1,2-epoxypropane. The technique increases the number of observable cells, when compared to preparations of planar monolayers, and there is good preservation of morphological detail.