Chromatin remodeling during spermiogenesis: a possible role for the transition proteins in DNA strand break repair
- 16 February 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 514 (2-3) , 111-114
- https://doi.org/10.1016/s0014-5793(02)02380-3
Abstract
An important chromatin remodeling process is taking place during spermiogenesis in mammals and DNA strand breaks must be produced to allow the accompanying change in DNA topology. Endogenous DNA strand breaks are indeed detected at mid-spermiogenesis steps but are no longer present in mature sperm. Both in vitro and in vivo evidence suggests that the DNA-binding and condensing activities of a set of basic nuclear ‘transition proteins’ may be crucial to the integrity of the chromatin remodeling process. We propose that these proteins are necessary for the repair of the strand breaks so that DNA fragmentation is minimized in the mature sperm.Keywords
This publication has 40 references indexed in Scilit:
- Targeted Disruption of the Transition Protein 2 Gene Affects Sperm Chromatin Structure and Reduces Fertility in MiceMolecular and Cellular Biology, 2001
- Teratozoospermia in mice lacking the transition protein 2 (Tnp2)Molecular Human Reproduction, 2001
- Haploinsufficiency of protamine-1 or -2 causes infertility in miceNature Genetics, 2001
- Transition nuclear proteins during spermiogenesis: Unrepaired DNA breaks not allowedMolecular Reproduction and Development, 2001
- Architectural DNA-Binding Properties of the Spermatidal Transition Proteins 1 and 2Biochemical and Biophysical Research Communications, 1998
- Damage to Rat Spermatozoal DNA after Chronic Cyclophosphamide Exposure1Biology of Reproduction, 1995
- Effect of Deoxyribonucleic Acid Protamination on Fluorochrome Staining and in Situ Nick-Translation of Murine and Human Mature Spermatozoa1Biology of Reproduction, 1993
- Deoxyribonucleic Acid Loop Domain Tertiary Structure in Mammalian Spermatozoa1Biology of Reproduction, 1993
- HMG 14 and protamine enhance ligation of linear DNA to form linear multimers: Phosphorylation of HMG 14 at Ser 20 specifically inhibits intermolecular DNA ligationBiochemical and Biophysical Research Communications, 1991
- Specific organization of genes in relation to the sperm nuclear matrixBiochemical and Biophysical Research Communications, 1990