Studies on the Presence of Adenosine Cyclic 3′:5′-Monophosphate in Oat Coleoptiles
Open Access
- 1 February 1975
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 55 (2) , 346-351
- https://doi.org/10.1104/pp.55.2.346
Abstract
The incorporation of adenosine-8-14C into adenosine cyclic 3′:5′-monophosphate in coleoptile-first leaf segments of Avena sativa L. was investigated. Homogenates of segments incubated in adenosine-8-14C for either 4 or 10 hours were partially purified by thin layer chromatography followed by paper electrophoresis. A radioactive fraction, less than 0.06% of the 14C present in the original homogenate, migrated as adenosine cyclic 3′:5′-monophosphate during electrophoresis. Upon treatment with cyclic nucleotide phosphodiesterase, however, less than 10% of this radioactive fraction appeared as 5′-AMP. Deamination with NaNO2 as well as further chromatographical purification also suggested that only a small fraction of the 14C in the partially purified samples could be in adenosine cyclic 3′:5′-monophosphate. The data suggest that levels of this nucleotide can probably be no greater than 7 to 11 picomoles per gram of fresh weight in oat coleoptiles. Treatment of such coleoptiles with physiologically active concentrations of indoleacetic acid, furthermore, had no significant effect on the 14C radioactivity in marker adenosine cyclic 3′:5′-monophosphate-containing fractions at any stage of purification during several experiments.Keywords
This publication has 16 references indexed in Scilit:
- Adenosine 3′, 5′-cyclic monophosphate in higher plants: Assay, distribution and age-dependencyBiochimica et Biophysica Acta (BBA) - General Subjects, 1974
- Formation of Adenosine 5′-Phosphorofluoridate and the Assay of Adenyl Cyclase in Barley SeedsPlant Physiology, 1974
- Occurrence and biosynthesis of adenosine 3′,5′-cyclic monophosphate in isolated Avena etioplastsBiochimica et Biophysica Acta (BBA) - General Subjects, 1973
- Evidence for the presence of 3′, 5′-cyclic AMP in plant tissuesBiochemical and Biophysical Research Communications, 1973
- Inability to detect cyclic AMP in vegetative or sporulating cells or dormant spores of BacillusmegateriumBiochemical and Biophysical Research Communications, 1973
- Auxin-induced synthesis of cyclic 3′, 5′-adenosine monophosphate in coleoptilesLife Sciences, 1971
- Effect of indole-3-acetic acid on the synthesis of cyclic 3′–5′ adenosine phosphate by bengal gram seedsBiochemical and Biophysical Research Communications, 1971
- MICROASSAY OF ADENOSINE‐3′,5′‐MONOPHOSPHATE (CYCLIC AMP) IN BRAIN AND OTHER TISSUES BY THE LUCIFERIN‐LUCIFERASE SYSTEM1Journal of Neurochemistry, 1971
- Influence of gibberellic acid on the incorporation of 8-14C adenine into adenosine 3′,-5′-cyclic phosphate in barley aleurone layersBiochimica et Biophysica Acta (BBA) - General Subjects, 1970
- Enzymic analysis of cyclic 3′, 5′-AMP in mammalian tissues and urineAnalytical Biochemistry, 1969