A RADIOIMMUNOASSAY FOR HUMAN MYOGLOBIN - LOWER DETECTION LIMIT, PRECISION, AND USE IN FOLLOWING THE COURSE OF MYOGLOBINURIC KIDNEY FAILURE

  • 1 January 1984
    • journal article
    • research article
    • Vol. 22  (1) , 61-64
Abstract
The lower detection limit of a radioimmunoassay for human myoglobin was determined by 2 precision from day to day methods (90% and 3 s methods), and by 2 precision in series methods (95% confidence range and the method of Markowetz et Munz). According to Markowetz et Munz, the lower detection limit (as a measure of sensitivity) is the lowest myoglobin concentration which, in 15-fold assays, shows no binding value in common with that of the next highest concentration, and no activity value in common with that of the reference binding value. On theoretical grounds (precision in series) and from a practical standpoint (determination of the lower detection limit using 1 sample assay), this method is the most suitable for the determination of the lower detection limit as a measure of sensitivity. It remains to be seen whether this a valid generalization for other methods.

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