Oestrogenic activity of subterranean clover. 2. The isolation of genistein from subterranean clover and methods of quantitative estimation

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Abstract
From the "chloroplast" fraction of subterranean clover (Dwalganup strain) 12 mg genistein per 100 g dry matter of clover was isolated. From fresh clover 369 mg genistein per 100 g dry matter was isolated. A method of estimation of genistein in plant material and a micro-method of detection of genistein in small amounts of plant material have been developed. Four subterranean clovers studied contain genistein, whereas four other species contained no genistein. The Dwalganup strain of subterranean clover contained 740 mg/100 g dry matter, sufficient to account for the oestrogenic activity of the plant and therefore probably responsible for the infertility in sheep grazing on subterranean clover pastures.

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