CYTOLOGY OF SACCHAROMYCES CERVICIÆ WITH ESPECIAL REFERENCE TO NUCLEAR DIVISION

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Abstract
The nucleus is very similar to that of many higher plants. In the process of budding it does not show evidence of division until the bud has attained almost full size. In the prophase the disperse chromatin granules become aligned along the radiating linin strands and join to give rise to the chromosomes. The chromosomes separate on the equator of the spindle, and half of them migrate through the isthmus into the bud. Reconstitution of daughter nuclei seems to be identical with the same process in higher plants. This study was made possible by a modification of technique which stained the nucleus without affecting the abundant metachromatic material. The yeasts were fixed in Bouin''s fluid and stained in iron-alum-hematoxylin without completely washing out the picric acid. This greatly decreased the affinity of the metachromatin for the stain.

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