Immobilization of Human Red Blood Cells

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Abstract
Human red blood cells were immobilized in an alginate network which was cross-linked with Ca2+. The immobilized cells were stored for longer than 5 wk at 4.degree. C in an isotonic buffered NaCl solution containing glucose, inosine, adenine and guanosine for energy supply and from the alginate network by dissolving the matrix with citrate. Both the immobilized and released cells retained their biconcave shape over the storage period. Measurements of the released cell population in a hydrodynamically focusing Coulter Counter demonstrated that the mean size of the size distribution, the breakdown voltage, and the internal conductivity did not changed in contrast to control measurements on red blood cells stored conventionally.

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