Antibodies in Normal Rabbit Serum That React With Tissue-Specific Antigens on the Plasma Membranes of Human Adenocarcinoma Cells2
- 1 February 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 56 (2) , 339-344
- https://doi.org/10.1093/jnci/56.2.339
Abstract
Indirect viable cell immunofluorescence studies revealed, on the plasma membrane of cultured adenocarcinoma cells from the human colon and rectum, a tissue-specifiC antigen that reacted with a component of normal rabbit serum. The use of G200 column chromatography and monospecific antisera prepared against class-specific heavy chains of rabbit immunoglobulin identified the reactive components in rabbit serum as IgM. Examination of normal and malignant cells from various human tissues by immunofluorescence and adsorption studies showed that rabbit serum reacted only with cells derived from the intestinal epithelium.Keywords
This publication has 0 references indexed in Scilit: