Allotypic Determinants on the Fab Fragment of Rabbit Aa Locus Negative IgG-Immunoglobulin

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Abstract
Immunization of rabbits with a locus negative IgG immunoglobulin molecules obtained from an Aa2Aa2 rabbit suppressed in utero with anti-Aa2 resulted in isoantibodies directed against the a negative IgG molecules. The allotypic specificities are located on the Fab fragment of IgG and are absent on the Fc fragment. Peptide maps of tryptic digests of the Fab and Fc fragments of Aa negative and Aa positive IgG revealed no differences between the Fc fragments but four to five peptide differences between the Fab fragments. Subgroups of rabbit γ chains are thus identified by antigenic differences in the Fd region and these subclasses appear to have identical Fc regions.

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