APPLICATION OF ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA) IN DIAGNOSIS OF ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 99  (2) , 288-293
Abstract
IgE and IgG antibodies aginst Af [Aspergillus fumigatus] were measured by ELISA [enzyme-linked immunosorbent assay] in sera from 15 patients with ABPA [allergic bronchopulmonary aspergillosis] and compared with antibody levels in 6 patients with extrinsic asthma and immediate-type skin reactivity to Af (prick skin test 3+ or 4+) but no other evidence for ABPA. The assay used polyvinyl microhemagglutination plates as a solid phase to adsorb antigens of Af and a double-antibody system of class-specific anti-human globulin and alkaline phosphatase-conjugated goat anti-rabbit globulin. IgE-Af and IgG-Af were significantly greater among patients with ABPA than in patients with asthma, suggesting that this assay may be used as an important diagnostic aid. The advantages of ELISA over radioimmunoassay are discussed in regard to early detection of an indolent pulmonary disease.