Uptake and accumulation of putrescine and its lethality in Anacystis nidulans.

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Abstract
The rate of uptake of putrescine by A. nidulans depended on the external pH and the extracellular concentration of putrescine. Accumulation of exogenous putrescine was also proportional to the concentration of putrescine in the medium, suggesting that putrescine uptake was not subject to cellular regulation. An equation was derived to test the hypothesis that putrescine accumulation was due to ion trapping. Comparison of the predicted and observed intracellular concentrations of putrescine under various conditions showed a close correlation in support of the hypothesis of ion trapping. Under conditions leading to cell death (e.g., 150 .mu.M putrescine, pH 9.8), the correlation did not hold as a result of leakage of accumulated putrescine.