An active cytochrome c oxidase that has no tightly bound cardiolipin

Abstract
Bovine and dogfish (Squalus acanthias) heart submitochondrial particles and cytochrome c oxidase (EC 1.9.3.1) were prepared. Biphasic Eadie-Hofstee plots from steady-state polarographic assays were obtained for both species. Phospholipid analyses indicated that cardiolipin was absent from this active dogfish enzyme.