96-Well Electroporation Method for Transfection of Mammalian Central Neurons
Open Access
- 1 November 2006
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 41 (5) , 619-624
- https://doi.org/10.2144/000112279
Abstract
Manipulating gene expression in primary neurons has been a goal for many scientists for over 20 years. Vertebrate central nervous system neurons are classically difficult to transfect. Most lipid r... Brainwashing Primary cells, such as T cells and neurons, can be extremely difficult to transfect, often requiring the use of time-consuming and sometimes costly viral-based methods. Buchser et al. ...Keywords
Funding Information
- Miami Project to Cure Paralysis
- Buoniconti Fund (DOD W81XWH-05-1-0061)
This publication has 11 references indexed in Scilit:
- Effective gene delivery to adult neurons by a modified form of electroporationJournal of Neuroscience Methods, 2005
- Pathological missense mutations of neural cell adhesion molecule L1 affect neurite outgrowth and branching on an L1 substrateMolecular and Cellular Neuroscience, 2004
- Rapid and efficient electroporation-based gene transfer into primary dissociated neuronsJournal of Neuroscience Methods, 2003
- Electroporation of primary neural cultures: a simple method for directed gene transfer in vitroHistochemistry and Cell Biology, 2002
- Techniques for gene transfer into neuronsCurrent Opinion in Neurobiology, 2002
- KDEL-Cargo Regulates Interactions between Proteins Involved in COPI Vesicle TrafficDevelopmental Cell, 2001
- Gene Transfer Methods for CNS Organotypic Cultures: A Comparison of Three Nonviral MethodsMolecular Therapy, 2001
- A versatile microporation technique for the transfection of cultured CNS neuronsJournal of Neuroscience Methods, 1999
- Developmental cues modulate GABAA receptor subunit mRNA expression in cultured cerebellar granule neuronsJournal of Neuroscience, 1993
- Differential inhibition of neurone–neurone, neurone–astrocyte and astrocyte–astrocyte adhesion by L1, L2 and N-CAM antibodiesNature, 1985