Purification and Characterization of Two Voltage-Dependent Anion Channel Isoforms from Plant Seeds
- 1 November 2000
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 124 (3) , 1181-1190
- https://doi.org/10.1104/pp.124.3.1181
Abstract
Mitochondria were isolated from imbibed seeds of lentil (Lens culinaris) and Phaseolus vulgaris. We copurified two voltage-dependent anion channel from detergent solubilized mitochondria in a single purification step using hydroxyapatite. The two isoforms from P. vulgaris were separated by chromatofocusing chromatography in 4 M urea without any loss of channel activity. Channel activity of each isoform was characterized upon reconstitution into diphytanoyl phosphatidylcholine planar lipid bilayers. Both isoforms form large conductance channels that are slightly anion selective and display cation selective substates.Keywords
This publication has 45 references indexed in Scilit:
- Identification of Porin as a Binding Site for MAP2Biochemical and Biophysical Research Communications, 1996
- The mitochondrial permeability transitionBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1995
- Permeation of hydrophilic solutes through mitochondrial outer membranes: review on mitochondrial porinsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1994
- Porin interaction with hexokinase and glycerol kinase: Metabolic microcompartmentation at the outer mitochondrial membraneBiochemical Medicine and Metabolic Biology, 1991
- Biogenesis of mitochondrial porin: The import pathwayCellular and Molecular Life Sciences, 1990
- Voltage gating in the mitochondrial channel, VDACThe Journal of Membrane Biology, 1989
- Inhibition of adenine nucleotide transport through the mitochondrial porin by a synthetic polyanionFEBS Letters, 1988
- Purification of VDAC (Voltage-dependent anion-selective channel) from rat liver mitochondriaThe Journal of Membrane Biology, 1983
- Reconstitution in planar lipid bilayers of a voltage-dependent anion-selective channel obtained from paramecium mitochondriaThe Journal of Membrane Biology, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970