Growth and pigmentation in genetically related Cloudman S91 melanoma cell lines treated with 3‐isobutyl‐1‐methyl‐xanthine and β‐melanocyte‐stimulating hormone

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Abstract
4 clonal sublines of Cloudman S91 melanoma cells, S91/mel, S91/13, S91/6 and S91/amel, were evaluated for changes in growth, pigment content and plating efficiency during and after treatment with a cyclic‐AMP phosphodiesterase inhibitor‐melanin‐stimulating agent, 3‐isobutyl‐l‐methyl‐xanthine (IBMX) plus β‐melanocyte stimulating hormone (β‐MSH) or IBMX alone. After combined treatment, increases in melanin content on day 3 were 48, 27, 11, and 2 pg/cell in the four cell lines respectively. In each case IBMX alone was less effective than IBMX plus β‐MSH. Doubling time increased and plating efficiency decreased with increased melanization. The increases in doubling time and decreases in plating efficiency were cell line dependent. The greatest rate of increase in doubling time and decrease in plating efficiency as a function of melanin content were seen in S91/amel, which produced the least pigment. The lowest rates of increase/decrease were seen in S91/mel, which produced the most pigment. Melanin pigment induced in the cells was classified as etimelanin by EPR determination. The differential response to induction of pigmentation makes these cell lines suitable models for comparative studies on the role of melanin in pigment cell biology.