Does Rice Ragged Stunt Virus Lack the Typical Double Shell of the Reoviridae?

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Abstract
In rice ragged stunt virus preparations negatively stained with uranyl acetate, particles corresponding to the B-spiked subviral particles (SVP) but not the double-shelled virions of fijiviruses were seen. The B spikes were broader at the base than those of fijiviruses, but otherwise the particles were similar in size and shape to fijivirus SVP. In thin sections of infected rice plants, large numbers of virus-like particles were seen; most resembled the SVP of fijiviruses (such as maize rough dwarf virus and oat sterile dwarf virus) but some appeared intermediate between these and complete fijivirus particles. Rice ragged stunt virus probably does not produce a regular outer shell as do the fijiviruses, although the broad-based B spikes may in some profiles have given the appearance of a continuous shell.