Production of Goat, Rat, and Guinea Pig Antisera to Murine IgE

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Abstract
The study of immunoglobulins (Ig)3 has been facilitated by the production of specific anti-Ig sera (1–4). For each of the six of the known classes and subclasses of mouse Ig, there exists a myeloma synthesizing the corresponding paraprotein, which has been used for the preparation of specific antiserum. Myeloma proteins have also been used to study the IgE class of antibodies in man (5) and in the rat (6), but to date no mouse IgE (IgEm) myeloma has been discovered. Nevertheless, one report has been published in which it was claimed that rat anti-mouse IgEm was produced (7) by immunization of Lewis rats with syngeneic peritoneal cells coated with IgEm; the mouse reagin has been adsorbed onto these cells by incubating them with mouse reaginic serum (MRS). However, in spite of extensive efforts, this procedure could not be reproduced in this laboratory.

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