Anti-bovine antibody in human sera as a cause of nonspecificity in enzyme immunoassay
- 1 June 1987
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 25 (6) , 987-990
- https://doi.org/10.1128/jcm.25.6.987-990.1987
Abstract
Much nonspecific activity in enzyme immunoassay is due to reactions between anti-bovine antibodies which are commonly present in human serum samples and residual calf serum in viral antigens. Calf serum is used in cultured cells which are used to produce viral antigens. Addition of bovine proteins to serum samples to be tested for viral antibodies reduces this activity.This publication has 4 references indexed in Scilit:
- Absorption of Protein and Protein Fragments in the Developing Intestine: Role in Immunologic/Allergic ReactionsPediatrics, 1985
- Effect of Early Immunization on Antibody Response to Reimmunization with Measles Vaccine as Demonstrated by Enzyme-Linked Immunosorbent Assay (ELISA)Pediatrics, 1984
- Enzyme-Linked Immunosorbent Assay and Radioimmunoassay in the Serologic Diagnosis of Infectious DiseasesThe Journal of Infectious Diseases, 1983
- ELISA methodology for polysaccharide antigens: Protein coupling of polysaccharides for adsorption to plastic tubesJournal of Immunological Methods, 1979